
GFCO STATEMENT ON TESTING
For the purposes of GFCO's gluten-free certification program, GFCO only uses test kits validated through AACCI or AOAC for gluten testing during initial certification, auditing, and point-of-sale surveys, as well as for confirmation of customer complaints and initiation of recalls. GFCO requires that manufacturers also use these test methods for ingredient and finished product testing. No test methods other than those listed are acceptable for meeting your testing requirements for ingredients and finished products. If you are interested in using a kit that is not listed under VALIDATED METHODS, contact the kit manufacturer to determine if or when they expect to complete an AOAC or AACCI validation.
For surface swabbing as part of cleaning verification or validation, manufacturers may use one of the rapid methods on this list, or a generic protein swab. GFCO does not accept ATP swab results for gluten cleaning verification or validation.
Testing Requirements:
Read your contract carefully for minimum testing requirements. Compliance with testing is mandatory for certification. Quarterly reporting of test results to GFCO is required. Send your testing logs to testing@gluten.org at the end of each calendar quarter. Due dates are January 10, April 10, July 10, and October 10.
Click here to see our general tips for testing.
| KIT | PART NUMBER | MANUFACTURER | ADDITIONAL INFORMATION | CONTACT INFO | WEBSITE | VALIDATION | RAW INGREDIENTS | FINISHED PRODUCT | SURFACE SWABBING | CIP/RINSE | VALIDATED MATRICES |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Agrastrip Gluten G12 | 10001995 | Romer Labs | LEARN MORE | office@romerlabs.com (636) 583-8600 | https://gfco.org/agrastrip-gluten-g12/ | AOAC PTM 061403 | X | X | X | X | ice cream, dark chocolate, cookies, bread, rice flour, stainless steel |
| EZ Gluten | 510EZG | ELISA Technologies | LEARN MORE | info@elisa-tek.com (352) 337-3929 | https://gfco.org/ez-gluten/ | AOAC PTM 051101 | X | X | X | X | |
| Gluten Tox Pro | KIT3000 (KT-5660) KIT 3001 (KT-5288) | Hygiena | LEARN MORE | customerservice@hygiena.com (888) HYGIENA (494-4362) (800) 863-6842 | https://gfco.org/gluten-tox-pro/ | AOAC PTM 061502 | X | X | X | X | bread, rice flour, pate, rolled oats, yogurt, painted wood, plastic, rubber, sealed ceramic, stainless steel |
| RIDAQUICK Gliadin | R7003 R7004 R7005 | R-Biopharm | LEARN MORE | info@r-biopharm.com (877) 789-3033 | https://gfco.org/r-biopharm/ | AOAC OMA 2015.16 AOAC PTM 101702 AACCI 38-60.01 | X | X | X | X | stainless steel, sealed ceramic, plastic, silicone rubber, clean-in-place rinse water |
| Neogen Gluten Protein Rapid Kit | L25GLU | Neogen Corporation | LEARN MORE | (800) 328-1671 | https://gfco.org/neogen-gluten-protein-rapid-kit/ | AOAC PTM 011601 | X | X | X | X | buckwheat, chocolate syrup, dry cereal, pasteurized soy milk, rice flour, bread dough, stainless steel, clean-in-place solutions |
| Neogen Reveal 3-D for Gluten | 8505 | Neogen Corporation | LEARN MORE | foodsafety@neogen.com (800) 234-5333 | https://gfco.org/neogen-corporation/ | AOAC PTM 122201 | X | X | quaternary ammonium-based clean-in-place rinse, stainless steel | ||
| OnSite Gluten Test Kit | PA-44 | Microbiologique | LEARN MORE | (866) 256-1804 info@onsitefoodsafety.com | https://gfco.org/onsite-gluten-test-kit | AOAC PTM 012501 | X | X | X | X | Rice flour, oat flour, spice mix, bread, stainless steel |

| KIT | PART NUMBER | MANUFACTURER | ADDITIONAL INFORMATION | WEBSITE | CONTACT INFO | VALIDATION | RAW INGREDIENTS | FINISHED PRODUCTS | SURFACE SWABBING | CIP/RINSE | VALIDATED MATRICES |
|---|---|---|---|---|---|---|---|---|---|---|---|
| RIDASCREEN Gliadin | R7001 | R-Biopharm | LEARN MORE | https://gfco.org/ridascreen-gliadin | info@r-biopharm.com (877) 789-3033 | AOAC OMA 2012.01 AOAC PTM 120601 AACCI 38-50.01 | X | X | Buckwheat, rice, corn, oats, syrup, sausage, maize dough, rice dough, wheat starch, rice flour, corn flour | ||
| Veratox Quantitative Gliadin R5 | 8510 | Neogen | LEARN MORE | https://gfco.org/veratox-quantitative-gliadin-r5 | foodsafety@neogen.com (800) 234-5333 | AOAC PTM 061201 | X | X | cooked hamburger, bread, rice cereal, rice flour | ||
| AllerTek Gluten | ELISA Technologies | LEARN MORE | https://gfco.org/allertek-gluten | info@elisa-tek.com (352) 337-3929 | AOAC OMA 991.19 AOAC PTM 081202 | X | X | Rice flour, cooked dough | |||
| AgraQuant Gluten G12 | 10001994 | Romer Labs | LEARN MORE | https://gfco.org/agraquant-gluten-g12 | office@romerlabs.com (636) 583-8600 | AOAC OMA 2014.03 AACCI 38-52.01 | X | X | Rice flour, chocolate cake, crisp bread | ||
| RIDASCREEN Gliadin Competitive | R7021 | R-Biopharm | LEARN MORE | https://gfco.org/ridascreen-gliadin-competitive | info@r-biopharm.com (877) 789-3033 | AOAC OMA 2015.05 AACCI 38-55.01 | X | X | Beer, starch syrup, sourdough | ||
| Morinaga Wheat/Gluten ELISA Kit | M2103 | Morinaga Institute of Biological Sciences | LEARN MORE | https://gfco.org/morinaga-wheat-gluten-elisa-kit | sales@crystalchem.com (630) 889-9003 | AOAC PTM 011804 | X | X | ice cream, soup, water, cider, rice flour, bread | ||
| SENSISpec INgezim Gluten R5 ELISA | 30.GLU.K.2 | Gold Standard Diagnostics Madrid | LEARN MORE | https://gfco.org/sensispec-ingezim-gluten-r5-elisa | Info.Spain@eu.goldstandarddiagnostics | AOAC PTM 052005 | X | X | X | bread mix, oat flour, stainless steel | |
| RIDASCREEN Total Gluten | R7041 | R-Biopharm | LEARN MORE | https://gfco.org/ridascreen-total-gluten | info@r-biopharm.com (877) 789-3033 | AOAC OMA 2018.05 | X | X | Oats | ||
| TotalTarget Kit for Gluten | 13025 | EnviroLogix Inc. | LEARN MORE | https://gfco.org/totaltarget-kit-for-gluten | (866) 408-4597 | AOAC PTM 032301 | X | whole corn, corn flour, corn grits, corn meal and soy | |||
| GlutenTox ELISA Rapid G12 Kit | KIT3075 | Hygiena | LEARN MORE | https://gfco.org/glutentox-elisa-rapid-g12-kit | customerservice@hygiena.com (888) HYGIENA (494-4362) (800) 863-6842 | AOAC PTM 042301 | X | X | spiked soy flour, corn bread, seasoning mix, rolled oats and evaporated milk; incurred baked bread |

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